The
@sloan_kettering
facilities staff have outdone themselves again with an extraordinary holiday display, and during pandemic! Wow!
#MSKKids
thank you!
#immigrantscience
I came to US with my parents as a refugee, naturalized and became US citizen. My lab has always been mostly immigrants on OPT, J1, and H1 visas. Exchange of ideas and people is an inherent part of science, and fundamental part of USA since inception.
New record for the lab (and not a good one). Revision of latest manuscript contains 79 new figure panels to address reviewers’ questions. To quote Hidde Ploegh: End the tyranny of reviewer experiments!
Human cancer is a disease of somatic mutations. As we age, tissues accumulate errors in DNA, but what causes mutations in young healthy cells? Read our new paper “Childhood cancer mutagenesis caused by transposase-derived PGBD5” 1/n
BIG ANNOUNCEMENT! Introducing the North American recipient of the St. Baldrick's 2018 Robert J. Arceci Innovation Award - Dr. Alex Kentsis!
See his work & how his research for better treatments for kids with cancer will leap forward – thanks to YOU:
TWEETORIAL to explain my lab's plan for the next few weeks (sent to my lab via email, and included here for others should it be helpful, modified as needed) 1/n
Comprehensive proteogenomics is here! Nearly complete detection of the majority of proteins and their complete sequences expressed by different human leukemias (using multi-protease digestion and multi-dimensional high-accuracy mass spectrometry)
Read our latest on the causes of oncogenic DNA rearrangements in medulloblastomas, a common childhood brain tumor: Childhood cancer mutagenesis caused by a domesticated DNA transposase
Are you interested in chemical biology, biochemistry and chemical proteomics? Well-known
#proteomics
secret: the majority of all recorded mass spectra currently remain unassigned. Read our latest manuscript by
@paolocifani
, Danmeng Luo, and Zhi Li on solving this problem. 1/n
Are you interested in oncogenic transposons, chromatin, and DNA nucleases? We are looking for ambitious post-doctoral research fellows: please join us! It's an exciting project!
Genome-wide screen in triplicate of a few billion leukemia cells? No problem says
@u2ma316
as he dispenses 10 liters of media in tissue-culture bags. Bravo! This one is especially for you
@TuomasTammela
Read our latest: chemotherapy resistance, leukemia stem cells, proteomics, CRISPR mouse engineering, and regulation of kinase-dependent control of pioneer transcription factors as improved therapy of AML. Many thanks to all the collaborators!
Together with
@KharasLab
@HellerLab
, we've made contingency plans for our labs
@sloan_kettering
, should more intensive COVID mitigation measures will need to be implemented, summarized below 1/n
Please read and use our latest work with
@CompProteomics
on agnostic chemical proteomics: Discovery of Protein Modifications Using Differential Tandem Mass Spectrometry Proteomics
Fascinating comparison of single-cell (stable) proteomes and (noisy) transcriptomes from
@labs_mann
@fabian_theis
et al. Ultra‐high sensitivity mass spectrometry quantifies single‐cell proteome changes upon perturbation.
@iannisaifantis1
It’s a problem that’s only getting worse, with only a few attempting to solve it
@eLife
, and what Hidde wrote a long time ago is still true:
Reviewed a manuscript for a prominent journal from authors I know but not friendly with: "This is an important study that should be published. The manuscript is well written and conclusions are well supported. My only request is that the data are made publicly available."
Excited about new logo. Though not completely intentional, the symbol combines DNA helix, ion beam, and caduceus, all of which are prominent in the lab.
Interested in epigenetic therapies? Read updated
@YKazansky
’s manuscript on Overcoming clinical resistance to EZH2 inhibition using rational epigenetic combination therapy, which shows that E2F-RB1 axis is a major mechanism of tazemetostat resistance
New faculty recruitment
@sloan_kettering
: Protein Systems Initiative (PSI) is a newly formed initiative to develop innovative proteomics technologies to propel transformative biological questions. 1/n
Sadly,
@ProPublica
and
@charlesornstein
have stooped to tabloid writing, at the expense of a life-saving
@sloan_kettering
institution that seeks nothing more than bringing knowledge and care to patients and science. Shame. On. You.
Impressive reporting, you got a transcript of people trying to work through an internal problem in a private setting. Hope you didn’t get a papercut in the process.
Quantitative Cell Proteomic Atlas: Pathway-scale targeted mass spectrometry for high-resolution functional profiling of cell signaling from
@paolocifani
and openly available at
Why do young people get cancer? I summarize a general framework of the causes of early‐onset cancer development in children and young adults by relating inherited and constitutional cancer predisposition, oncogenic pathogens, and developmental mutations.
We posted an update to "A transposase-derived gene required for human brain development" including additional patient families with PGBD5 deficiency syndrome and mouse model studies for its unanticipated (neuron genome remodeling!) mechanism of action:
Interested in why young people get cancer and how to develop new therapies to target them? Read
@anton_gh
and our new JCI Insight - Emerging functions of DNA transposases and oncogenic mutators in childhood cancer development
Are you interested in cancer biology and pediatric oncology? I will be presenting
@AlexsLemonade
as part of their Register to receive a link for August 19:
@timothykeyes
It’s a marathon, not a race. Focus on fundamental problems that will leave a long-lasting impact on people and fields. No amount of money or “accomplishments” beats this.
@chorye
@JSheltzer
That’s the best answer so far, e.g. tumor cells tolerate aberrrant expression of oncogenes and loss of tumor suppressors, but normal cells cannot. Same for loss of BRG1 or SMARCB1...
Today: a morning of caring for sick children with inspiring nurses and NPs, mid-day of interviewing stellar MD-PhD applicants, afternoon of discussing new science with passionate lab fellows, evening of writing new grant proposal and manuscript.
#nofilterneeded
Glad to see this work from
@NovartisScience
building on our probes to target MYB! Design and Biochemical Characterization of Peptidic Inhibitors of the Myb/p300 Interaction Clinical-grade inhibitors next!
@lstops
1) Manage goals, not tasks. 2) Begin every meeting with questions about person, not project. 3) Recruit people who will complement you, not replicate you.
Read our latest, led by inimitable
@LuzJubierre
, defining the physiologic functions of PGBD5: A transposase-derived gene required for human brain development
Fantastic work by
@sloan_kettering
colleagues. Diet-regulated production of PDGFcc by macrophages controls energy storage . Makes one wonder if mutant macrophages in people with clonal hematopoiesis have altered fat regulation and energy balance?
@DrSidMukherjee
Sid, I would include a discussion of the causes of cancer in young people as opposed to those due to aging and environmental exposure. This includes inheritance of cancer-predisposing mutations and aberrant activity of endogenous mutators, such as RAG1/2 in lymphoid cancers.
First in-person group meeting since spring 2020. What a breath of fresh air! Great science, great discussion, and such a delight to speak with everyone!
We are (carefully) beginning to resume our lab research: if you are interested in a Position for a Post-Doctoral Research Fellow in Functional Cancer Genomics - we are looking for new colleagues ☛
Another beautiful study from Neal Rosen and colleagues on blocking oncogenic BRAF fusions: RAF inhibitor PLX8394 selectively disrupts BRAF dimers and RAS-independent BRAF-mutant-driven signaling.
In these stressful times, I am sending books to students, technicians and fellows in my lab, here they are in no particular order, hoping they will bring spark, resolve, inspiration or just a smile. Enjoy and share your books and tips!
Look at this
@TODAYshow
of the awesome holiday display by
@sloan_kettering
plumbers, electricians, painters, mechanics and other outstanding facilities support staff to brighten up
#MSKKids
For kids undergoing treatment at Memorial Sloan Kettering Cancer Center, the holidays are a whole lot brighter this year thanks to a group of employees with hearts of gold.
Absolute tour de force by Sumiko Takao in the lab! 10L lentiviral culture->functional genetic library transduction of patient AML cells->labeling and dox-induction in transplanted mice->multicolor FACS to isolate and sequence leukemia quiescent stem cells to identify regulators.
Preparing to submit
@ReviewCommons
revision of our manuscript on oncogenic gene control in AML and grateful to Sumiko Takao,
@u2ma316
, Shuyuan Cheng and many colleagues for the heavy lift during the COVID pandemic to finish this work.