Osvaldo Contreras
@osvaldoics
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Cell Identity + Cell Cycle Networks + Chromatin Dynamics & DevEvo in Human Development 𧬠@UNSWMedicine | #MyoTwitter π @Prelights @PddcSydney #eLifeAmbassador
Sydney, New South Wales
Joined May 2019
Because #OpenScience cannot wait...π Excited to share our latest preprint: ππ©ππ§ππππ! π¬ ππ©ππ§ππππ: a Versatile, Open-source EdU Multiplexing Methodology for Understanding DNA Replication and Cell Cycle Dynamics π‘ Affordable, efficient, and user-friendly, ππ©ππ§ππππ enhances EdU click chemistry staining with off-the-shelf reagents, enabling high-quality imaging across various cell types and complex 3D structures. π Validated in multiple cell types, embryos, adult organs, hiPSC-derived organoids, and zebrafish larvae our work opens new avenues for understanding development, growth, and DNA replication. π #OpenEMMU #DNAReplication #CellCycle #OpenSourceScience #FluorescentImaging #FlowCytometry
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RT @AusCellDeathSoc: (1/4) πAs promised, 2025 is here, and weβre kicking it off with something big! Introducing the ACDS 2025 Mentorship Pβ¦
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RT @slavov_n: Todayβs most transformative medicines exist because of fundamental discoveries that were made without regard to practical outβ¦
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RT @TanentzapfLab: 1. A viable scientific ecosystem provides a variety of environments for scientists to thrive. Some need to be left aloneβ¦
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RT @Nature: Nature research paper: Engineered heart muscle allografts for heart repair in primates and humans
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RT @osvaldoics: 17/ π¦π Finally, we labeled nascent DNA in one of my favorite animal models, 5dpf zebrafish larvae, and visualized it not onβ¦
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RT @osvaldoics: 16/π«π Using recent and advanced tissue clearing methods (e.g., DEEP-Clear; PMID: 32523996, DISCO-like technique) and 3D ligβ¦
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RT @osvaldoics: 15/ π¬ We also demonstrated OpenEMMU's compatibility across cutting-edge iterative multiplex immunolabeling techniques (e.g.β¦
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RT @osvaldoics: 14/ π As we are interested in how embryonic development occurs and is controlled, we challenged OpenEMMU for spatial biologβ¦
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RT @osvaldoics: @StVHealthAust 13/ π¨OpenEMMU not only facilitates high-parameter flow cytometry but also advanced imaging techniques, suchβ¦
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RT @osvaldoics: 12/ 𧬠Apart from working with bone marrow cells (which actively proliferate and self-renew) and splenocytes, we employed Opβ¦
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RT @osvaldoics: π To address these limitations, we developed OpenEMMU: 8/π₯ΌOpenEMMU is a flexible (detailing labeling protocols and reagentβ¦
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RT @osvaldoics: 1/ 𧬠5-Ethynyl-2'-deoxyuridine (EdU) has revolutionized DNA replication and cell cycle analyses with its fast and efficientβ¦
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RT @osvaldoics: Because #OpenScience cannot wait...π Excited to share our latest preprint: ππ©ππ§ππππ! π¬ ππ©ππ§ππππ: a Versatile, Open-sourceβ¦
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Happy New Year to all of my friends and colleagues who celebrate this date! ζεεθ΄’ (GΕng xΗ fΔ cΓ‘i), δΈδΊε¦ζ (WΓ n shΓ¬ rΓΊ yΓ¬)!
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