Retrieving the complex responses of individual cells in the native three-dimensional tissue context is crucial for a complete understanding of tissue functions. Here, we present PHYTOMap (Plant...

We summarize recent progress and share our perspective in the areas of extracellular immunity, sensing of immunogenic signals by cell surface and intracellular immune receptors, heterogeneity of...

Plants protect themselves from herbivores by optimizing the distribution of chemical defenses

Nature Plants - The study describes the development and validation of Multi-Knock, a genome-scale clustered regularly interspaced short palindromic repeat toolbox that overcomes functional...

Nature Biotechnology - Protein structure prediction with a language model improves accuracy for orphan and designed proteins.

Proteolytic cascades regulate immunity and development in animals, but these cascades in plants have not yet been reported. Here we report that the...

Nature Reviews Chemistry - Optical and electrochemical sensing techniques have been used to detect real-time chemical signals in living plants in response to biotic and abiotic stress. Through...

Nature Genetics - This Perspective on crop genomics discusses the promises of new technologies and approaches to help minimize food insecurity and to lay the foundation for sustainable agricultural...

Cell-surface peptide-receptor complexes RGF1-RGFR and Pep1-PEPR function as pH sensors to regulate extracellular pH-mediated growth and immunity in root apical meristem region in Arabidopsis.

Nature - Missing component found in the pathway that closes leaf stomata.

Nature Biotechnology - Plant traits are fine-tuned by stepwise downregulation of gene expression.

The bHLH Ia/IIIb regulatory module plays a key role in stomata development. Chang et al. suggest that bryophyte SMF transcription factors possess functions partially similar to AtMUTE and AtFAMA. The...

Nature Plants - This Perspective reviews recent advances in crop transformation technologies that promise to lead to the widespread adoption of genome editing in crop species.

Nobori Lab @TSL

Nature - Fusion of rice Pong transposase to the Cas9 or Cas12a programmable nucleases provides sequence-specific targeted insertion of enhancer elements, an open reading frame and gene expression...

Nature Biotechnology - Rice and potato plants are more productive after epitranscriptome engineering.

A plant immunity protein interacts with a fungal cell wall–degrading enzyme to create immunogenic compounds.

Nature - Tamlo-R32, an engineered wheat mutant allele of the Mildew resistance locus O (MLO) gene, confers resistance to powdery mildew, retains robust wheat growth, and can be transferred to other...

In plants, the types and amplitudes of defense outputs against bacterial and fungal pathogens depend on cellular sugars.

Zhou et al. observe that the within-plant “up-down” movement of predatory mites on wild potato plants switches their feeding behavior from palynivory to carnivory. This movement behavior is mediated...

A single bifunctional protein links nitrate sensing to changes in transcription in the small mustard plant Arabidopsis thaliana.

Identification of cis-regulatory dynamics in the maize genome at single-cell and cell-type resolution by single-cell sequencing of assay for transposase accessible chromatin.

Abstract. Gene functional descriptions offer a crucial line of evidence for candidate genes underlying trait variation. Conversely, plant responses to envi

Plants emit species- and stress-specific airborne sounds that can be detected in acoustic chambers and greenhouses.

Nature Genetics - A study of 580 tomato lines resulting from a cross between a wild desert-adapted species and a domesticated cultivar elucidates the genetic basis of gene expression and metabolite...

Nature - A genetically encoded sensor for the quantitative visualization of auxin distribution in plants enables real-time monitoring of its uptake and clearance by individual cells and within...

Plants maintain populations of pluripotent stem cells in shoot apical meristems (SAMs), which continuously produce new aboveground organs. We used ...

Nature Biotechnology - Programmable and reversible CRISPRi-based genetic circuits function in a variety of plants.

A high-quality rice pan-genome of genetically diverse rice accessions is constructed through de novo genome assemblies, demonstrating the impact of structural variation and gene copy number variati...

Extreme environmental conditions, such as heat, salinity, and decreased water availability, can have a devastating impact on plant growth and produ...

Nature - A plant endogenous peptide-receptor signaling pathway termed SCREW–NUT is described; it counteracts microbe-associated molecular pattern (MAMP)- and abscisic acid-induced stomatal...

Nature Communications - Here, Jin et al., develop a method called Barcoding Bacteria for Identification and Quantification (BarBIQ), which allows to both characterize the global microbiome and to...

Nature Plants - A massively parallel reporter assay was used to measure the activity of nearly complete sets of promoters from Arabidopsis, maize and sorghum in two assay systems, uncovering the...

Plant TIR domain-containing proteins are bifunctional enzymes with NADase and cyclic nucleotide monophosphate synthetase activity. Combinations of these activities may cooperate to control cell death...

Nature Plants - Huang et al. develop a potent genome editing toolkit to generate transgene-free genome-edited plants in the T0 generation by co-editing of ALS gene (without obvious fitness costs)...

This review discusses the features of cis-regulatory sequences in plants, technologies enabling their identification, characterization, and validation, the

Uncovering the basis of small-molecule hormone receptors’ evolution is paramount to a complete understanding of how protein structure drives functi...

A ligand deprivation–dependent activation system helps plants tune stress responses near damaged sites.

Plants utilize a two-tiered immune system consisting of pattern recognition receptor (PRR)-triggered immunity (PTI) and effector-triggered immunity...

Nature Biotechnology - Challenges and opportunities for engineering and studying the soil microbiome are discussed.

This study reports on developing the first nitrate sensor visualizing nitrate dynamics at a spatiotemporal cellular resolution.

Nature - Evolutionary conservation of plant receptor structure allowed for generation of new variants of wheat and barley nucleotide-binding leucine-rich repeat receptors (NLRs) that recognize...

Exchange of small RNAs between host plants and their pathogens modulates gene expression in the interacting partners during host-pathogen interactions. Wang et al. show that the model plant Arabido...

Nature Microbiology - Systematic analysis of bacterial phyllosphere isolates and their ability to protect Arabidopsis thaliana plants against infection, with the model pathogen Pseudomonas syringae...

Nature - Cryo-electron microscopy structures of a 2.8 megadalton bacterial type IV secretion system encoded by the plasmid R388 and comprising 92 polypeptides provide insights into the stepwise...

We provide an update on recent advances in our understanding of SA metabolism, perception, and signal transduction mechanisms in plant immunity.

Nature Plants - The authors developed a platform for rapid identification of interacting plant immune receptors and pathogen avirulence proteins by library screening in protoplasts, then used it to...

Nature Chemical Biology - Methyl jasmonate in the root volatile organic compounds (rVOCs) signals to the soil microbiome to form biofilms with altered composition that benefits plant growth. This...

Extensive studies of the reference plant Arabidopsis have enabled deep understandings of tissues throughout development, yet a census of cell types and states throughout development are lacking....

Iron-limiting soils are widespread, causing significant losses in plant growth and productivity. Harbort et al. show that under iron limitation, plant-secreted coumarin compounds are mediators of a...

Successful plant pathogens produce a suite of small molecules called effectors that are injected into plant cells and perturb plant defense. As a counter defense, plants have evolved intracellular…

Nature Plants - Balance between growth and stress response is critical for plants to adapt to high salinity. This study shows that receptor-like kinase FERONIA takes advantage of phyB-mediated...

Nature Microbiology - The comparison of the ability of native and engineered gene clusters transferred into bacteria that live on or inside cereal roots to regulate nitrogenase activity reveals...