Molecular Horizons Cryo-EM
@UOWcryoEM
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The Molecular Horizons CryoEM Facility at the University of Wollongong. Microscopes include Tecnai T12, Talos Arctic 200 kV and Titan Krios 300 kV.
University of Wollongong, Australia
Joined May 2019
@WimJHH Would love to hear some advice in this space. Our pixel size is slightly off and it would be great to get some 'best practice' hints from experts.
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@Struc_biol_PL @bluesocks81 @thermosciEMSpec Also curious, why the low pixel count and large Å/pix? Looks like K3 with binned pixels? Multiple shots per hole as well? When you say ‘optimal optics’, can you clarify?
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Amazing opportunity for an industry-focussed PhD! Join the @arc_gov_au Industrial Transformation Training Centre for #CryoEM of Membrane Proteins! Multiple positions - @MolHorizons
@Bio21Institute
@WEHI_research
@ihmri
@MIPS_Australia
@MonashUni
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Panasas press release about our strategic alliance:
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This should be interesting. More competition for direct electron detectors.
After 8 years of intense R&D, we are thrilled to announce "Apollo" -- the world's first event-based electron counting direct detector for cryo-EM. Learn more and register for a Webinar at #finally #wishlist #cryoem #electronmicroscopy
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@3rdFloorTom @BJ_Greber @cryo2go @benjaminbarad I’d expect higher resolution for aldolase. We once hit 2.5 A with our Arctica/F3 with <100 mics. And regularly get under that (2.2-2.3 A) on our Krios/K2 with ~500 - 1000 mics. This is with minimal processing.
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@CorneliusGati @ImagingArtifact We found a 6x 2080Ti was a good balance. A couple of fast CPUs and lots of SSD.
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This three paper expose in Nature Communications has now been picked up by the editors and featured in "Structural biology, biochemistry and biophysics". Nice work teams! @MolHorizons @uonmolmicro
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Novel RNA-Polymerase molecular mechanism that allows pathological bacteria to maintain gene expression discovered here at UOW CryoEM @MolHorizons @gokhan_tolun in collaboration with Uni of Newcastle @uonmolmicro. This work is published in Nature Comms.
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RT @DawidZyla: Another great tool to visualize #CryoEM data! I recently found @napari_imaging: a python package that allows viewing multidi…
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@biochem_fan @RadoDanev Totally agree. In our facility we have found real time processing makes data collection much more efficient. Screening data can be tested rapidly for quality, rather than collecting for hours on poor grids.
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RT @RadoDanev: High defocus does not attenuate high-resolution components.🌞 An excellent study by Bob Glaeser and colleagues.🤩 For good sam…
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Check out some of the latest research from our facility! Arctica + Cryosparc + Krios 👌 @MolHorizons @uowresearch @thermosciEMSpec Molecular Basis for RNA Polymerase-Dependent Transcription Complex Recycling By The Helicase-like Motor Protein HelD.
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@bluufive @DustinMorado We have also tried this approach. Refine the Cs based on nominal pixel size, then use the ratio of ‘refined’ Cs to actual Cs to calculate the actual pixel size.
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Just over a week after arrival in Wollongong @UOW the Krios is now fully operational at Molecular Horizons @UOWcryoEM @MolHorizons. It passed the first image tests with flying colours, and we are looking forward to seeing what it can do!
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@CloudyKielkopf @MolHorizons @thermosciEMSpec @structurabio They were your grids Claudia! Nice work with the amazing grid prep.
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