Kenneth W Witwer (he/him)
@KennethWWitwer
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Following
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I study extracellular vesicles, enveloped viruses, and noncoding RNA at Johns Hopkins University; President of ISEV, host of EVClub, and consultant
United States
Joined April 2013
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@MicrobiomDigest
I have known several lab members whose mere presence seems to kill our cell cultures. This explains so much...
6
4
229
I’ve often wondered: is it possible to forget your jacket in Baltimore and get one for $5.99 at a second-hand shop in Jacksonville the night before your talk? It is, my friends, it is!
7
1
178
With sincere apologies...couldn't resist!
#TypesOfPapers
#ExtracellularVesicles
see original
@xkcd_rss
@IsevComms
@LabWitwer
13
23
159
Good…good…the Marriott has put up the decorations as requested!
#ISEV2022
#ExtracellularVesicles
#EVcoutrements
6
3
155
I have an amazing team who shine in the toughest times & look out for me even when they’re hurting, too. We have all gone through a lot with the death of our lab manager this week, who took care of us and our research for 20+ years. We will miss you, Brandon, and we will carry on
25
1
144
Small
#ExtracellularVesicles
include but are not limited to
#exosomes
. Exosomes are EVs from the
#endosomal
system.
#Ectosomes
/
#microvesicles
can be just as small as
#exosomes
. Let's please be careful not to equate
#sEVs
with the
#exosomal
subset.
#PublicServiceAnnouncement
1
32
132
Setting up a Zoom meeting? Avoid the
#darkside
of
#zoombombing
: use a password, disable screen sharing, annotation, and whiteboard, and allow only the host to unmute and enable sharing. See the FBI warning here
@NPR
5
90
113
Announcing the
#EXCEL
#EVCore
Facility in the
@LabWitwer
@HopkinsMedicine
. We can help you separate, characterize, and functionally study
#ExtracellularVesicles
#EnvelopedViruses
#Nanoparticles
with the most rigorous technologies available.
Briefly, EXCEL offers:
Particle
7
24
126
Look what's under the tree:
#MISEV2023
has been accepted for publication! This third iteration of the international consensus guidelines on minimal information for studies of
#ExtracellularVesicles
is brought to you by
@Joadwe
@lodrisc1
@GoberdhanD
& Clotilde Théry, plus the
6
57
157
Chairs and vice-chairs of the Gordon Research Conference on
#ExtracellularVesicles
:
@EditBuzas
@EstherNolteHoen
#PaulRobbins
&
@LabWitwer
lots of work but rewarding to bring 200 people together for excellent
#EVScience
!
@IsevComms
@GordonConf
@HopkinsMedNews
0
3
113
VERY exciting work on
#ExtracellularVesicles
and
#SARS_CoV_2
coming out of
@institut_curie
with
#ClotildeThery
#NicolasManel
@lmartinjaular
et al
@train_ev
@IsevComms
#COVID19
#WatchOutVirusHereEVsCome
2
41
105
If you didn't make it to
#ISEV2023
, you missed several tons of great science...but don't despair: the
#EducationDay
talks are now freely available on our YouTube channel!
@IsevOrg
@IsevComms
#EVClub
2
41
110
There continues to be substantial confusion about what is in a prep of
#ExtracellularVesicles
, like
#ectosomes
and
#exosomes
. Here, the Clotilde Théry group found that ectosomes actually outnumber exosomes in some preps.
6
27
108
Let’s remember to give each other credit. The
#undergrad
who had a good idea. The
#technician
who worked hard every day to make a paper possible. The
#mentor
who taught you and gave you a chance to thrive. You don’t lose by giving credit.
#Publication
#SciencePublishing
2
10
103
People ask me if there’s one instrument to phenotype, size, and count
#ExtracellularVesicles
. Glad to announce the “Ken-o-scope” that works at least for macro-sized EVs 🤣
#joking
#ServiceForHire
6
3
106
A plea from Cherie Blenkiron: let’s stop publishing the same figure over and over again referring to “three types” of
#ExtracellularVesicles
: artificial distinctions that belie the true diversity of
#EVs
!
#MoreThanExosomes
#MoreThanMicrovesicles
5
14
105
#ElectronMicroscopy
shows us some amazing things. Just what are those
#multilamellar
#ExtracellularVesicles
we sometimes see, and how do they arise? Thanks
@WolframJoy
& collaborators for sharing these insights and speculation!
@ISEV_Journals
@IsevOrg
1
19
103
Small
#ExtracellularVesicles
#sEVs
including
#exosomes
, small
#ectosomes
seem to get all the love 🥰...what about larger
#EVs
like
#exophers
#LargeOncosomes
#MicroParticles
#Mitovesicles
? Also important! Are you interested in a Workshop on Large EVs? DM me!
12
12
91
Beautiful to be reminded from time to time how lucky I am to have the most amazing lab and collaborators
@LabWitwer
#EVBirthday
#HappyVesicles
@ArabTanina
@OlesiaPhd
VassoMachairaki
@Troymen11
@Blankette
@bonita_powell
@MartaGarciaCon2
@Yiyao_Huang
24
0
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2010: year of
#Instagram
#Wikileaks
#Anonymous
...hey, I loved it, too, but now it's 2022 & not just
#microvesicles
&
#exosomes
anymore...check out this masterly review by
@EditBuzas
@IsevOrg
on the amazing menagerie of
#ExtracellularVesicles
in
#immunology
2
12
85
#JournalImpactFactor
of
#JournalofExtracellularVesicles
is now 25.841. What does this mean? Like any metric, IF is imperfect and can be misused, but it does mean that influential papers are published in the journal. Please send JEV your best work!
#ExtracellularVesicles
@ISEV_JEV
1
19
89
First time in my career that a paper was accepted on first submission and without revisions! Also cool that it was the second paper accepted today…a rare good-news day, and I’ll gladly take it! 😅
6
1
87
Extracellular vesicles versus synthetic nanoparticles for drug delivery...thanks
@WolframJoy
@ChristineHorejs
@NatRevMater
@MayoClinic
@HopkinsMedicine
@LabWitwer
Comment on
#ExtracellularVesicles
#nanomedicine
#drugdelivery
#EVs
0
20
85
Seeking a motivated postdoc to join our dynamic team. Study
#ExtracellularVesicles
incl EV release, uptake assays, novel methods to separate EVs and other exRNA carriers. Must be able to start in Sept/Oct. COVID means local candidates/in the US may have preference. Send CV to me.
1
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82
Fantastic time in
#Seattle
with the world’s experts on
#ExtracellularVesicles
#ISEV2023
. Here’s a few, and me pretending to know where I’m going 😂
@LenassiMetka
@hillandy
1
0
76
"The big news is that the society has now decided to create a new
#journal
":
#ExtracellularBiology
w/
@hillandy
as Editor-in-Chief, announces
@IsevOrg
President Clotilde Théry at the
#ISEV2021
#GeneralAssembly
. This journal has a broader focus than
@ISEV_JEV
#ExtracellularVesicles
1
22
73
I bet most of us have never screened 27 DIFFERENT BUFFERS!! Amazing work from
@andregorgens
and El-Andaloussi lab on storage and stability of
#ExtracellularVesicles
, presented now on
#EVClub
@IsevOrg
@IsevComms
@ISEV_JEV
3
6
73
Having a bit of a fun
#debate
on
#miRNAs
and other
#RNAs
in
#ExtracellularVesicles
(or not!)…do
#EVs
transfer miRNAs functionally? Are full-length
#mRNAs
found in EVs? I think we need an event devoted to this topic someday!!
@ISCTglobal
@IsevOrg
7
10
75
Are
#EV
#miRNAs
wimps or warriors? Our
#EVJournalClub
will soon discuss the
@biorxivpreprint
everyone's been talking about Be sure to sign up at , date and time soon
@lambertlab1
@LabWitwer
@exRNA
@IsevComms
@WebEVTalk
@janlotvall
13
21
73
I don't care what it looks like, its an EXOSOME!!!
9
1
70
Realizing the tremendous potential of
#ExtracellularVesicles
requires the best available methods. Check out this Nature
@MethodsPrimers
piece from
@AnHendrix2
et al for up-to-date info.
2
20
72
My crucial competencies by scientific career stage
Undergraduate: washing glassware, learning basic techniques
Graduate: experimental design and building a coherent publication
Postdoc: integrating projects, mentorship, and networking
PI: reducing manuscript wordcounts by 33%
2
1
69
Good to ponder! Some possible urban legends in my field:
1) There are three types of
#ExtracellularVesicles
2)
#EVs
are well known to cross the
#BloodBrainBarrier
in both directions
3) EVs are non-immunogenic
4)
#miRNAs
are the main cargo of EVs
@IsevComms
@LabWitwer
@IsevOrg
The phenomenon of reviews citing reviews citing reviews with no sign of the original publication of a finding after looking at the 3rd review makes me wonder how much of science is just urban legend.
#citeprimaryliterature
7
17
280
3
5
66
Did you know that tumors can be hot? As in, up to 3.5°C hotter than surrounding tissue? AND temp affects release of
#ExtracellularVesicles
! Check out this recent ms from Otsuka, Yamamoto, and
@IsevOrg
board member Takahiro Ochiya:
@IsevComms
#cancer
#EVs
5
13
71
The latest
@LabWitwer
preprint is up
@biorxivpreprint
. Here, we characterize
#ExtracellularVesicles
and artificial nanoparticles with several technology platforms. What's best? Well, no single technique can do everything; good to use orthogonal methods!
4
16
66
Nooooo! Or did I??
4
8
70
Calling all experts on
#ExtracellularVesicles
: your input is requested by Friday, August 26 on the next
#MISEV
(minimal information for studies of EVs) coordinated by
@IsevOrg
@lodrisc1
@GoberdhanD
@Joadwe
How can you get involved?
4
25
69
Here is
@MartaGarciaCon2
presenting her
#EVTAG
technology to
#ISEV2022
#FeaturedAbstract
@IsevOrg
@IsevComms
#ExtracellularVesicles
@LabWitwer
#Tetraspanins
@JohnsHopkins
9
6
69
Here's a free-of-charge link to the recent
@naturemethods
manuscript on imaging of
#ExtracellularVesicles
in vivo from
@frwkie
@guillaume_niel
and the
@IsevOrg
#ISEVImaging
workshop
0
18
66
miRafficionados, miRskeptics, miRcurious! So much left to learn about
#ExtracellularVesicles
and
#miRNAs
! Please join
@doldivizio
,
@LabWitwer
& Albanese and Pegtel, Monday 1 June 10 am EDT(GMT-4) Preprint here: Sign up for login at:
2
18
69
5
14
67
No commentary needed! 🤣 fun science and interactions at the Gordon Research Conference on
#ExtracellularVesicles
@GordonConf
@hillandy
@IsevComms
0
2
67
Brain-derived
#ExtracellularVesicles
in
#Alzheimers
had more pronounced differences in cell-enriched markers and other proteins than brain tissue
@Yiyao_Huang
@tomdriedonks
@JHPrecisionAlz
@hillandy
@bonita_powell
@ArabTanina
@labwitwer
#MesoScale
1
12
64
I don't often tout numbers, but this shows the devotion & involvement of the
@IsevOrg
#EVResearch
community: we just hit 3000 (!) subscribers to the
#ISEV
#EVClub
YouTube channel...join here for more on
#ExtracellularVesicles
#ectosomes
#exosomes
1
7
66
I have a joke about circular RNAs, but it just never ends...
0
2
60
Huge interest in
#ExtracellularVesicles
: >1700 signed up for
#EVClub
in the last year. How did they learn about it? Here's the breakdown. To join and learn more about
#EVs
, visit ...and if you're already on board, keep sharing!
@IsevComms
@LabWitwer
#ISEV
1
16
64
Happy Friday, everyone! What better time than now for a walk through the pages of
#EV
history? Courtesy of
@dr_yvonne_couch
#DaveCarter
@hillandy
@doldivizio
@SusmitaSahooPhD
@janlotvall
et al
#ExtracellularVesicles
2
21
60
The Gordon Research Conference on
#ExtracellularVesicles
takes place July 24-29, 2022 in beautiful Newry, Maine. Apply early...limited spots for this GRC! I co-chair with Paul Robbins and vice-chairs
@EditBuzas
@EstherNolteHoen
@GordonConf
@LabWitwer
1
14
64
Really damn cool:
@DanielBachurski
used tellurium-phenylalanine to do mass cytometry and check different
#SEC
fractions to determine
#EV
dependent, independent cellular uptake
#ISEV2022
#ExtracellularVesicles
@GSEV10
@IsevOrg
@IsevComms
2
8
61
Thanks to all who made 9 months of
#EVJournalClub
possible: all presenters, moderators, participants,
@LabWitwer
members! Want to join us in 2021? Sign up here and subscribe to
#ExtracellularVesicles
@IsevComms
#HappyNewYear2021
2
12
63
Loved this conversation with
@janlotvall
on
#SARSCoV2
#Covid_19
#HIV
and whether the enveloped
#virus
is an
#exosome
#ectosome
. And how do
#viruses
and
#EVs
enter the cell? Can
#ExtracellularVesicles
be used as
#Vaccines
and therapies?
@IsevComms
@exRNA
2
10
61
The Journal of Extracellular Vesicles
@ISEV_JEV
, yes, has a new impact factor. More importantly and impressively in my view: on Clarivate's list of 20,000 journals, JEV is the
#4
ranked journal with 100% open access content.
#ExtracellularVesicles
#ISEV
2
6
61
Huge problem: false positives from lipid dyes in studies of
#ExtracellularVesicles
! Learn about a solution on
#EVClub
: water-soluble conjugated oligoelectrolytes (COEs). Wed Mar 15 8PM EDT/5PM Pacific/8AM Beijing Need the login?
2
3
61
I'm so proud of my team
@LabWitwer
for pulling together to make things happen. After a rough couple of years, I realized today that we have more primary research papers under review than ever before...and more on the way. Thank you All!!
0
0
63
You know what I love? Talking about science with people in my lab! Or, for that matter, with people anywhere!
#MoreScienceLessNonsense
2
3
61
We have a
@YouTube
channel for the
@LabWitwer
#EVJournalClub
, and we do our best to reply to comments and questions! 🤣
2
3
61
What's the connection of
#autophagy
,
#ExtracellularVesicles
and
#exRNA
? Join
@tomdriedonks
@LabWitwer
on Wed April 1 via to discuss a very cool paper from
#JayDebnath
lab
@UCSF
@UCSFPathology
#EVJournalClub
@exRNA
@HopkinsMedNews
#LC3
#RNA
@JohnsHopkins
4
29
56
3
2
60
Going to
#ISEV2023
? The Link light rail is the cheapest way to get from SeaTac airport to the conference: only $3. Grab your bags, go to 4th floor, skybridge to the parking lot, and follow the signs, easy-peasy!
7
5
59
Whoa…80% of the participants in this poll at
#ISEVxTECH
are using
#SEC
#SizeExclusionChromatography
for
#EVSeparation
#ExtracellularVesicles
@IsevComms
2
9
57
A reviewer recently ruled "reject" on a submitted paper since we measured small
#ExtracellularVesicles
<100 nm in diameter by
#NanoFlow
. In the reviewer's opinion, this finding was invalidating, since "classical extracellular vesicles have a size of greater than 100 nm."
In
8
6
57
Anyone looking for a postdoc position doing crazy stuff with
#ExtracellularVesicles
? Message me for more info...
@IsevComms
6
21
56
Thanks JP...Lessons I learned: always ask who's paying the bills, and never let anyone put words in your mouth
Why would a scientist retract his own opinion piece in a journal like Nature? Thanks
@KennethWWitwer
for this example of scientific integrity.
1
7
39
1
6
57
Short break at the Gordon Conference on
#ExtracellularVesicles
in beautiful
#Maine
…great to see old friends and make new ones after four long years!
@GordonConf
@IsevComms
#science
#scienceHikeBalance
1
0
56
Really enjoyed sharing my enthusiasm for
#ExtracellularVesicles
with the audience at
#TermisEU2022
@TermisEU2022
@IsevComms
thanks
@MathieuBrunet_
for the pic!
1
2
56
Grateful that Katalin Kariko
@kkariko
could join
#ISEV2022
to educate
#EV
researchers on the development of
#mRNA
#therapies
! An example to follow for
#ExtracellularVesicles
!
@IsevComms
@IsevComms
#vaccines
#COVID19
0
10
55
Abstract submissions are now open for
#ISEV2021
in
#Lyon
: Start thinking about yours today...deadline is January 15. We hope to meet in person, but it will be a landmark meeting no matter what!
#ExtracellularVesicles
#EVs
@IsevOrg
@IsevComms
2
18
55
Announcing the
#ISEVxTech
#Technology
and
#Methods
Summit, 16-18 November, 2022. Save the date and stay tuned for details on this important meeting at the cutting edge of science on
#ExtracellularVesicles
! Hope to see you there
@IsevOrg
@IsevComms
#ectosomes
#exosomes
0
12
54
Way more abundant than
#ExtracellularVesicles
in blood:
#Lipoproteins
. Learn about these
#EV
cousins at the next
#EVClub
@JohnsHopkins
by Dr. A. Zivkovic
@ucdavis
on Wed April 15, noon EDT. Not signed up for the link? See here:
@IsevComms
@exRNA
@LabWitwer
0
17
54
No one better to learn from about
#ExtracellularVesicles
than Clotilde Théry herself! At the
#CanVesicle
symposium, she reminds us that
#ectosomes
and
#exosomes
can be the same size and reviews the various methods for
#EV
#separation
.
#Canada
#EVs
@IsevComms
0
13
56
Exercise your brain next week with a jam-packed
#EVClub
: SEVEN groups share their work on
#ExtracellularVesicles
and
#exercise
Need the login?
@IsevOrg
@ISEV_Journals
@IsevComms
@LabWitwer
0
17
55
Well, this is definitely cool:
@jptosar
's work on
#exRNA
featured in this week's edition of
@Nature
@IsevComms
@IPMontevideo
@Udelaruy
0
9
56
Are
#ExtracellularVesicles
the same between brain regions?
@ArabTanina
presenting at
#ISEV2023
@IsevOrg
@LabWitwer
and thanks to chairs Ursula Sandau and
@SettyMagana
0
8
55
A perennial question on EVeryone's mind...and new answers with
@andregorgens
: join
#EVClub
next Wednesday to learn about optimal
#storage
of
#ExtracellularVesicles
! Note the different time For the login:
@IsevComms
@ISEV_JEV
0
7
55
Signage is up and
#ISEV2022
is all set to begin! Thanks Steph for the pic and to the whole
#IOC
for their hard work in making this happen, especially chairs Lorraine O’Driscoll
@lodrisc1
and Sophie Rome
#ExtracellularVesicles
#ISEV
@IsevOrg
@IsevComms
1
3
54
"Quantification of
#ExtracellularVesicles
in vitro and in vivo using sensitive bioluminescence imaging" recently published in
@ISEV_JEV
0
11
51
Report from
@Yiyao_Huang
@ArabTanina
and colleagues on preparations of
#ExtracellularVesicles
from different brain regions is now out in
#InterdisciplinaryMedicine
@WileyGlobal
@LabWitwer
2
13
53
Do you want to get more attention for your latest work on
#ExtracellularVesicles
? Announcing the
@IsevOrg
#EVClub
trailers, a short, straight-to-video format. Just ignore the corny jokes...the info is accurate!
@IsevComms
@LabWitwer
2
18
52
1
1
50
Huge proud co-advisor congrats to Bonita Powell
@bonita_powell
for passing your oral qualifying exams!! Next stop PhD!! We’re all so happy for you and tbh not too surprised at the outcome!
@BCMB_JHMI
7
4
48
This is THE BIG ONE! The largest and most comprehensive
#ExtracellularVesicles
event so far. Abstract submissions open shortly for
#ISEV2020
in
#Philadelphia
May 20-24 2020
@IsevOrg
@IsevComms
@ISEV_JEV
@exRNA
#MISEV
0
21
48
WHERE DO THE EVs GO?? Join
#EVClub
and
@SNEVResearch
this week for "Pharmacokinetics and biodistribution of EVs administered IV & intranasally"
@tomdriedonks
&
@jianglinglei
w/
@Willhotham
Wed Oct 20, noon EDT/18.00 CEST
#ExtracellularVesicles
@IsevComms
2
14
47
Wow! What a surprise for my birthday! Zach and
@Ashley_Russellx
lured me into the conference room to discuss some
#ExtracellularVesicles
experiments with
@IdylleLabs
dyes, and suddenly everyone cheering with balloons, cake, and presents! (We talked about the experiments...later)
15
0
50
Curious about
#supermeres
and
#exomeres
? Next
#EVClub
covers a recent exchange in
#JExBio
&
Need the login?
w/
#BobCoffeyLab
@jptosar
et al! 4 the login:
@LenassiMetka
@ISEVComms
1
12
50
Volunteering this morning.
@Moveable_Feast
helps People living with
#chronicdisease
#HIV
#foodinsecurity
by delivering nutritious meals. Here’s
@Blankette
and me from
@LabWitwer
and thanks
@DWWilliamsLab
for organizing! 1900 meals prepped.
0
4
48
Sequences that drive packaging of
#miRNAs
into
#ExtracellularVesicles
...what's the evidence? Don't miss discussion@ next
#EVClub
Jan 26:
@Marc_Halushka
presents, authors have been invited. 4the login:
@IsevOrg
@IsevComms
#ExoMotifs
@Nature
#sEVs
#microRNAs
1
7
51
Our latest is now in J Extracell Vesicles: Characterization of
#EVs
and synthetic nanoparticles with 4 orthogonal single‐particle analysis platforms. Twitter asks if I want to read the article before posting...believe me, I have read it!
#ExtracellularVesicles
@IsevComms
@LabWitwer
new publication is up on the
@ISEV_JEV
website
It is my great honor to contribute as co-first with
@emilymallick
. Many thanks to the outstanding lab mates and collaborators
@KennethWWitwer
@Yiyao_Huang
@OlesiaPhd
@IsevComms
@JohnsHopkins
5
5
19
2
9
48
3rd talk at the
#ISEV2022
#EducationDay
is from
@guillaume_niel
on
#labeling
and
#imaging
of
#ExtracellularVesicles
. Guillaume is also president of
@French_Soc_EVs
.
@IsevOrg
@IsevComms
see also the recent publication from
@frwkie
and
#ISEVImaging
3
4
47
and so you see, they reported "exosome" transfer when it was really "label" transfer
0
2
51
The
#ISEV2020
meeting will now be
#virtual
. Sad to miss you all in person, but happy to catch up online. PLUS it's not just 3 days: access to digital content continues for 60 days after the event!
@IsevComms
#ExtracellularVesicles
@IsevOrg
3
25
49
What can
#CryoEM
tell us about
#ExtracellularVesicles
? What are those weird "EVs in EVs" we observe? Real? Artifacts? Join next
#EVClub
for more!
Need the login?
@ISEV_Journals
@IsevOrg
@LabWitwer
@NatalieTurnerAu
@WolframJoy
1
12
49
Many thanks to the
#ISEVxTECH
for actualizing the vision of an
#EVTechnology
meeting…it’s been a great experience! Special thanks to
@carney_lab
@cblenkie
!
@svploeg
@Joadwe
@GeneQuan
@LenassiMetka
2
11
49
Finally...the
#emoji
we’ve been waiting for:
#EVs
!! Thanks
@3prms3
for the heads-up. No more typing out
#ExtracellularVesicles
in WhatsApp etc
@IsevComms
@LabWitwer
1
9
49
A bacterial extracellular vesicle-based intranasal vaccine against
#SARSCoV2
#ExtracellularVesicles
#OMVs
#vaccines
#COVID19
@jianglinglei
@tomdriedonks
@OlesiaPhd
@sabraklein1998
@andrewpekosz
@IsevComms
@LabWitwer
@HopkinsMedNews
@JohnsHopkinsSPH
2
16
46
Here is Alissa Weaver
@AWeaverLab
presenting the IMPRESSIVE work of the
@VU_EVresearch
Center…truly a model for collaborative research, from training to events to funding and publications!
#ExtracellularVesicles
@VU_EVresearch
3
6
46