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Matthew Taliaferro
@JMTali
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RNA biologist @CUAnschutz @rnabioco @CU_BMG
Colorado, USA
Joined May 2011
@IgorUlitsky It’s intrinsic to the RNA and not the prep because reprepping the RNA sample doesn’t fix it. No idea what’s causing it. Probably ~30% of our samples are affected.
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RT @ShechnerLab: It's my pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope…
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@ewjwallace @HYG_Lo @RnaRae @joelle_lo @CUAnschutz @CUDenver @chrmosimann @SpitaleLab It used to be even worse. In development we had a metric we were optimizing called PORC (proportion of relevant conversions) but that didn’t make it into the final manuscript.
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@HYG_Lo @RnaRae @joelle_lo @CUAnschutz @CUDenver @chrmosimann @SpitaleLab Excitingly, we detect G conversions in the RNA from these embryos, and these conversions also show spatial specificity as MALAT1 had more conversions with a nuclear Halo fusion and GAPDH had more conversions with a cytoplasmic Halo fusion. [fin] <-- (zebrafish joke)
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