Bento Lab 🧬
@theBentoLab
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We make Bento Lab = a portable PCR workstation for scientists and educators. Tweets about methods, field sequencing and DNA barcoding
London
Joined October 2013
PCR workstation with laptop-sized footprint? ✅ 32-well thermocycler? ✅ 8,000 × G centrifuge? ✅ Electrophoresis unit? ✅ Blue light transilluminator? ✅ Spare capacity/fieldwork/teaching/at home? ✅ Affordable and portable✅ Check out Bento Lab here:
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@S4ngano You could maybe try a gel extraction using 6M sodium iodide to dissolve the agarose and cause the DNA to bind onto silica particles, such as ? ADB buffer from Zymo has sodium iodide in it according to the SDS sheet so it's probably something similar.
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RT @theBentoLab: Want to teach DNA barcoding but not sure where to start? We’ve rounded up 4 lesson plans packed with practical steps — fr…
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@Roninatty Interesting - I imagine that might work quite well in a pinch. An electric one like an InstantPot?
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👌😂 echoes of Paul Vanouse doing Sauna PCR in Finland
@theBentoLab Will try at some point "even more extreme" ways: - using the heat disipated by a car radiator - using one tea light - using somehow a magnifying glass and solar light - using the heat disipated by a motorbike exhaust - using a capped jar exposed to sun in the mid summer
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RT @MonashTS: One of the most impressive pieces of equipment we have are @theBentoLab boxes. These devices function as a portable molecular…
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Want to teach DNA barcoding but not sure where to start? We’ve rounded up 4 lesson plans packed with practical steps — from field sampling to phylogenetics — designed for high school & undergrad classrooms. Learn more: Pictured: undergraduate students using Bento Lab at University of North Dakota! 📷 by Monica Norby.
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If you know of other studies comparing sequencing costs and success rates for ONT and other sequencing technologies, or if you have any thoughts on this topic, please let us know! You can find the article here: Cuber, Piotr, et al. "Comparing the accuracy and efficiency of third generation sequencing technologies, Oxford Nanopore Technologies, and Pacific Biosciences, for DNA barcode sequencing applications." Ecological Genetics and Genomics 28 (2023): 100181.
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Find more, including a guide to 4 ideas for PCR practicals for undergraduates or high school students, on our website here:
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You can find the article here: Chen et al. (2023). A home-made pipette droplet microfluidics rapid prototyping and training kit for digital PCR, microorganism/cell encapsulation and controlled microgel synthesis. Scientific Reports, 13(1), 184. You can read more about ddPCR here:
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Open access! You can find the article here: Ivanova & Kuzmina (2013). Protocols for dry DNA storage and shipment at room temperature. Molecular ecology resources, 13(5), 890-898.
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