Ophir Shalem
@ophirshalem
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Assistant Professor, Department of Genetics, University of Pennsylvania, Children's Hospital of Philadelphia
Philadelphia, PA
Joined April 2010
Excited to share our new paper where we explore why dCas9-KRAB protein levels drop so drastically after neuronal differentiation in WTC11 and KOL2.1J iPSC lines.
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Huge congratulations to former lab postdoc @YSereb for starting his own independent lab! Make sure to follow him for exciting future science and check out his lab if you are looking for opportunities in the NJ/NY area!
The Serebrenik Lab is hiring! Join us in unraveling proteostasis with pooled protein tagging and manipulation! Interested postdocs can learn more & meet other excellent labs at the Rutgers Postdoc Peek (Apr 3-4, 2025). Apply by 1/8/25:
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Fascinating work from Jongens and @ErikaHolzbaur lab demonstrating that FMRP granules mark mitochondrial fission sites in axons and dendrites and serve as sites of local translation!
Incredibly proud of the beautiful work from Adam Fenton!
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RT @nevillesanjana: 🚨🚨🚨 Thrilled to share our newest study — identifying functional long noncoding RNAs using transcriptome-scale RNA-targe…
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I would also like to thank four editors for desk rejecting this brief communication, still looking for a home if any editors around
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@AbellJonny I think v possible, but testing localization is a good idea, happy to send you the exact sequences we used and plasmids to test
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@AbellJonny That is interesting, In which lines did you not see the levels go down after differentiation? We tested WTC11 and KOLF. Was it always dCas9-KRAB(KOX1)?
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Excited to see the revised version of our paper now published at @CellGenomics led by @YSereb
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Excited to share this collaboration spearheaded by @GMBurslem lab! Part of our long term goal of developing tools for scalable and non-disruptive perturbation and imaging of the endogenous proteome!
Ever wanted to edit a protein sequence inside a living mammalian cell? Maybe to site specifically incorporate a non-canonical residue in a cell? Perhaps you’d like to do a pull down without an antibody? Or use a small molecule fluorophore to image an endogenous protein?
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Such a fun evening with bittersweet goodbyes and welcoming new members. Real privilege to work with so many talented scientists. Make sure to follow @stephsansbury @YSereb @Saranya to find out about the next steps in their careers!
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RT @mikejg84: New in @ScienceMagazine: Our paper mapping gene/isoform/splicing regulation at pop-scale during human #neurodevelopment. Maj…
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RT @metaBailism: Why do activated T/B cells wait to divide? Why is there heterogeneity in their daughter cell responses? How do we find pea…
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