RT @erblabs: We are looking for a postdoc - in a project on a UbiD like (de)carboxylase!

RT @mirbachh: Lets talk about #acetyl-CoA! As central molecule in C1 metabolism, the efficiency of its assimilation determines yields. Read…

@NicoC_MicSynBio Congratulations Nico!

@WeiDu13 @erblabs @mpi_marburg And as a rough comparison/estimation, we routely cultivate E coli with only 10 mM glucose, which is for all the biomass/energy requirements, 0.5mM Thr should be enough to relieve auxotrophy due to the thrB knock out.

@WeiDu13 @erblabs @mpi_marburg For the Q2, nope, 0.5mM Thr should be enough. The strain also couldn't grow even when complemented with thrB from a plasmid (Fig. S9, black & blue lines).

@WeiDu13 @erblabs @mpi_marburg Thank you! For the Q1, mutations happened naturally/randomly, but the knock-out strain grew under selective conditions. We interpreted this to be a lower selection pressure when with PN. Fig4 indicated that 13nM could partially relieve selection.

RT @SebWenk: 🚨 Job alert 🚨 Please retweet 🚨

@schulluc Congratulations!

@NicoC_MicSynBio @ERC_Research @WUR @MIB_WUR Congratulations

RT @mirbachh: With #glyoxylate as core intermediate of synthetic #carbon fixation cycles, coupling #growth to its availability is essential…

RT @mirbachh: Harnessing the high product titers from fermentations for aerobic productions? Metabolic rewiring makes that possible! Read b…

RT @simone_giaveri: Super excited to share my main research @erblabs, new in @ScienceMagazine, where I engineered a cell-free system that s…

RT @AMKueffner: Happy to share my first pre-print out of my post-doc in the @erblabs at the @mpi_marburg. If you are If you are interested…

Happy to share our curated medium-scale E coli metabolic model out. We believe it has many application potentials, a few demonstrations shown in our preprint check it out. Great job by Marco! #Synbio #metabolism @WeTheBarEvenLab

RT @rene_inckemann: Very excited to share the preprint of a big chapter of my PhD, where I am corresponding & shared first author (together…

RT @enricoorsi: Obtaining efficient #catalysts is one of the core challenges of #bioengineering. However, combination of existing #SynBio,…

RT @SebBarthel: I am very happy to share our pre-print on the prototyping of a complex enzyme cascade (the CETCH cycle) using in vitro tran…

RT @mpi_marburg: What an exciting discovery! You all know fractals, like romanesco, snowflakes, or fern leaves? In the molecular world, fra…

RT @NicoC_MicSynBio: A recent preprint from our lab about MOSAIC: a very easy protocol to make (short) mutations or libraries in plasmids m…