Roland Hatzenpichler
@environmicrobio
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10,367
PhD / archaea.exobiology.methane.single cell physiology / for evidence-based decision-making / I hold strong opinions / posts reflect only my own views
Bozeman, MT
Joined December 2015
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Pinned Tweet
My lab is always looking for enthusiastic and driven postdocs to join the lab. If you are in the EU and want to spend some time working on novel methanogens in beautiful Bozeman, MT, consider applying for a Marie-Curie fellowship, email me your ideas!
Methanogenesis outside the Euryarchaeota experimentally demonstrated by three cultivation-driven studies (two from my lab)! A long🧵.🐻with me
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Please refuse to work for free for for-profit publishers.
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To the PIs publishing in Nature Microbiology. How do you tell your first author student that you just paid a for-profit company 1/3 of the student’s annual salary (costs are $10,186 per open access article) to make a 6 page pdf? I am genuinely interested in their reaction.
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Disappointed by the ABC books for Toddlers when it comes to (micro)biology because they get stuff wrong or focus on immunology. So I made a ‘ABC of microbiology’ for my son. U can it download from my lab website. My 16-month-old now can say ‘archaea’ 😍
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Could someone explain to me how they justify paying 5700$ to publish in Nature Comm? How do you get funding for this? My lab is well funded and I couldn’t afford these costs. And don’t funders complain? For that money, I can publish 2 OA ISME papers or 3 AEM papers...
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I hear a lot of people say “now is the chance to get that big person in the field to give talk; we couldn’t get in person”. No! Now is the time to give talks to postdocs/faculty with kids who typically cannot travel and highlight the work by as diverse researchers as possible!
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I have a personal story to share. This week was BIG for me. I received my first shot of an enzyme treatment of an inborn metabolic disease, phenylketonuria (PKU, ). If untreated, PKU eventually leads to
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I find the “please suggest alternative reviewers” the most absurd. Like, dude, this is your entire job! I won’t do your work for you.
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After 8 years of speculation: 2 preprints – 1 by my group , 1 by group of Lei Cheng – report 1st-time cultivation of methanogens outside the Euryarchaeota! Work in my group was led by
@kohtzarchaeota
. Funding by
@NASAAstrobio
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I just wanna raise again the issue how insane it is that US profs don’t get 12 month salary. The justification of this exploitive system is that we don’t teach in summer. True, but classroom teaching is like 20% of my workload. So why don’t I get 80% of my paycheck in summer?!
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@KristenClarkeJD
Virtually everyone in this video is black. This clearly shows who those Republican voter suppression laws are targeted at. Go vote them out!!!!
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Methanogenesis outside the Euryarchaeota experimentally demonstrated by three cultivation-driven studies (two from my lab)! A long🧵.🐻with me
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Meet the newest (identified) organelle, the nitroplast!
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How I published 2 research papers in my six years as postdoc: raised my own salary via 3 fellowships, had an original idea, worked hard and did the work myself, didn’t rely on people leaving work to write up, realized nrs of papers and citations are a poor indicator of success.
How I published 13 first-author papers during my 2.5 year post-doc:
- All the data were already collected
- 5 papers were literature reviews
- I read incredibly fast
- I write even faster
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And suddenly I am associate prof w tenure :) Feels anticlimactic. Should I treat myself? (Excuse to get a new boardgame...?)
In a shocking twist of events, I was recommended for tenure by the department. [now I just have to wait 7 more months till it went all the way up the food chain]
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In a shocking twist of events, I was recommended for tenure by the department. [now I just have to wait 7 more months till it went all the way up the food chain]
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@hannes_elefant
I get that. In my lab, the average person spends 12-14k per year. But that money is used to fund the ACTUAL science, not to pay a company to put a pdf on a website and handle some emails between four or five people.
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And yes, I understand that some funders/universities will cover the costs at no cost to the PI or grant, and, yes, I understand it’s not my business to tell you where to publish in.
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I am also looking for 1-2 students who wanna join the lab via the Microbiology (or Biochemistry) graduate program. Contact me for more info but succinctly, the students would continue our work on thermophilic methanogens. More info in my recent talk
Why earn a Ph.D. in Microbiology & Cell Biology at Montana State University?
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To be abundantly clear: genomic prediction =/= physiologically manifestation. Co-occurence in 16S dataset =/= symbiotic relationship. Two genes for porhphyrin synthesis in a MAG =/= phototrophy. Rubisco genes =/= carbon fixation. Small genome =/= small cell.
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A bacterial enzyme makes CH4 from methylamine. Big congrats to Qian Wang & Tim McDermott
@montanastate
to this great study! Thanks for involving my lab in later stages of this work. We now collaborate heavily. Funded by
@NSF
@JGI
@msuchembiochem
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My lab has so many exciting results, the next months will be a tour de force in paper writing: new archaeal lineages from hot springs, cultures of several novel methanogens, new correlative microscopy methods, new ways for phenotype-based cell sorting... so excited to share soon!
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Physiology ... is an emergent property that cannot be reliably predicted from genome data or metabolic reconstructions alone
#mytwocents
#metagenomics
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2 for 1 😊 Our article on Next-generation physiology approaches is live! We discuss cutting-edge approaches to non-destructively study cellular phenotype, separate those cells, and study them using downstream analyses! Thanks a lot for inviting us to write this
@NatureRevMicro
Next-generation physiology approaches to study microbiome function at single cell level
@environmicrobio
@microspietz
@jay_zackary
@msuchembiochem
@Center4Biofilm
@montanastate
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Please share! My lab is looking for a postdoc to join our lab! The postdoc’s objective will be to experimentally test genomic predictions on Asgard archaea physiology cultivation-independent, single cell resolving approaches. Candidates with microscopy XP are highly desired!
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@markabelan
Say hello to multicellular magnetotactic bacteria. Cartoon by
@SchaibleGeorge
. See
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Any faculty who still wear masks in all meetings, seminars and classes? Doesn’t look anyone is doing it here.
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A 6 page pdf that you wrote yourself and that people who read it for scientific quality weren”t paid to help to assess.
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Please share! I expect to hire 1-2 new grad students (+1 postdoc?) to start fall 2024 to work on novel methanogens in three (!) archaeal phyla. Culture based & in situ work in Yellowstone! Previous work: , & preprint coming 2morrow
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Suprised & honored that my
@NIH
@NIGMS
MIRA proposal was funded on 1st try! As a lab that has no xp with gut microbiology & in collab w. the
@MetrodoraInst
, we gonna throw all our fancy tools & new, to be developed ones at human colonoscopy (& some stool) samples. Will hire soon!
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I continue to be somewhat disappointed by
@nanopore
. Is this normal? New kit, fresh supplies, pretty clean samples but portion of good pores goes down rapidly. Is this normal?!
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Very excited that the
@GordonConf
on Applied and Environmental Microbiology was approved. We are scheduled for July 13-18, 2025 in South Hadley, MA! The associated GRS will be on July 12-13. Our fearless leader will be
@DeniseAkob
, assisted by
@Thuls_M
and myself.
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FREE STUFF! Did you know making your own tyramides for HRP/TSA reactions is 79x cheaper than buying, even considering labor? Well, my grad student, Stavros Trimmer, made $40,000 worth of fluorescein-conjugated tyramide for just $600 and now we enough for 1.25M reactions.
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@StephenNehrenz
Isn't the whole idea of having 2 of them to not have them in same place? Security and such?
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Congratulations to Anthony
@kohtzarchaeota
on his first first-author paper now online at
@ISMEComms
. Anthony discovered a new class of archaea in geothermal habitats & named them Culexarchaeia. Funded by
@NASA
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The most idiotic reviewer comment I ever got was “your method section is too long” yes, because we believe in reproducibility and WANT people to use our protocols!
Remember kids, if someone says your paper has too many details and that you should remove them, there's a poor grad student trying to replicate results from a publication with barely any information
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Congrats
@reichart_nick
on 1st 1st-author paper of your PhD
@ISMEJournal
. He used BONCAT-FACS to study microbes changing activity in response to incubation conditions. Complementary, conceptually different approach to D2O-RACS. Funded by
@NASAAstrobio
@NSF
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I heard people say a method is only truly recognized once it makes it into textbooks. I am excited that BONCAT-FISH & -FACS made into new ed. of “Brock Biology of Microorganisms”. BONCAT-FISH image credit also goes to
@MicroSpietz
&
@SchaibleGeorge
, which sadly are not mentioned.
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Are you a postdoc candidate with great ideas on exobiology or extreme life and need a lab to host you and give you access to deep sea and hot spring samples? Contact me with your ideas about applying for NASA's astrobiology postdoc felloship program!
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Please share! My lab is looking for a postdoc to join our lab. The postdoc’s objective will be to experimentally test genomic predictions on Asgard archaea physiology and their interactions using cultivation-independent, single cell resolving approaches.
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Looking for a postdoctoral researcher to work on Asgard archaea ecophysiology via a 3-year collaborative project funded by the
@SimonsFdn
! Please share widely.
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@kamil_gorecki85
Please report that person to the funding agency. Should not be allowed to review
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Was recently invited to two profs houses. It’s crazy what difference generational wealth makes and how people are oblivious to it and just assumes every other person or at least faculty can live like that. As a first gen college student, it’s amazing to me I bought a house!
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@LisaMariaN91
My take on this from my “grad student guide” i give to every new lab member or possible recruit.
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@Nature
Let’s give all our postdocs bonuses with the money we save by not publishing in Nature.
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@surt_lab
Here’s my math for a grad level class showing that clonality is a myth, should be applicable to your problem.
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@PhD_Genie
Never worn anything else at a conference or departmental seminar for that matter. Why would I? It’s not a fashion show. People should wear whatever they are comfy it, be it a t shirt or a suit. Noone should care about anything but the science.
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Covid positiv. I couldn’t have done anything to be more cautious. In past two weeks only went to store 3x times and 1x to library. No meetings outside household. Always masked. Used hand sterilizer. Triple vaxxed. Stay safe everyone!
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Discussed the 'arsenic life disaster' in grad class today and, just as every year, students were confused, surprised, shocked, dismayed, and then disillusioned (about the peer review process). Arguably the most important (of the 17) paper we discuss in class this semester.
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Attention! This new paper used data of a few PIs including myself without permission!!
@JGI
user violation Contacting authors and editor immediately!
@ScienceAdvances
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Turns out we missed a source of CH4 in oxic environments. And it’s bacterial and we know the enzyme! Originally discovered by our collaborators Tim McDermott and Qian Wang (out in PNAS soon), learn more by my grad student Will Christian
#WorldMicrobeForum
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An important reminder that all bioinformatics precitions must be benchmarked by wet lab experiments.
@JedFuhrman
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Reminder on Earth day that majority of O2 is made by cyanobacteria & algae & that trees are rather unimportant & that the idea of blaming individual choice for the problem is a systematic strategy by the industry and politicians to deflect responsibility. Still, do your best!
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@GovernorBullock
Make face masks required in all public settings! - signed, a microbial ecologist (PhD)
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After screening 100 hot springs,
@microbe_mack
and Viola Krukenberg, studied 12 MAGs from 3 metagenomes and tested their metabolic hypotheses using mesoscosms. In a 1st, they provide indirect evidence that archaea outside Euryarchaeota grow as methanogens
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Three papers showing methanogenesis by three members of the Thermoproteota (TACK) are out in Nature today: (figure by
@Kohtzarchaeota
who 1st authored both papers from my lab; 1 shared with Viola Krukenberg)
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Earlier this summer, my lab was awarded a FICUS, and now we received a large scale CSP! Thanks
@jgi
and
@EMSLscience
for your trust which will allow us to continue and expand our work in Yellowstone! Looking forward to working with JGI on this!
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Nominations for the Leeuwenhoek Medal, the highest honor in microbiology, given once every decade, are open. I have a hard time thinking of any single person (but it takes a village!) who transformed microbiology more than
@BanfieldJill
in last 10-20 years
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Am I the only one sick and tired of seeing omics-only studies be glorified by journals, but the experimentalists being told “not novel/interesting/sexy” enough?
Dear editors, the novelty of our study lies in fact that, in contrast to 95% of other studies, we actually tested our hypotheses from genomics and not just move to next metagenome. The fact that our experimental results confirm those hypotheses is, as far as I c it, irrelevant
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Final version of our correlative microscopy manuscript published in
@ISMEComms
by
@SchaibleGeorge
@Kohtzarchaeota
&
@EMSLscience
John Cliff. Get the most info from your cells by directly correlating their identity, activity, morphology, and chemistry!
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Nice summary of our lab’s new grant. Hiring soon for jobs to start in 2023. This work is only possible through a collaboration with the
@MetrodoraInst
and generous support from the
@NIH
@NIGMS
.
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Several colleagues and friends and I r up for tenure this semester. Since we all put so much work into putting all together, I thought I share the tenure package CV on my lab's website so it can serve as template to others (not sure if it's well prepared)
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@ChristiansenJen
I had a tshirt depicting this since I was 16. For many years, I hated to always be asked if I am a fan of Joy Devision. When I started my postdoc at Caltech, on the first day I was told they loved that pulsar data. That’s when I knew I was at the right place.
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@galosgann
I am totally on board with offering my review services to any society journal or publisher who gives money to a society. But I am unwilling to provide free service to a cooperation that exploits my labor to make money from it.
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Seeing is believing! Microscopic and metatranscriptomic analyses revealed unique cross-domain parasitism between phylum Candidatus Patescibacteria/candidate phyla radiation and methanogenic archaea in anaerobic ecosystems
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Ok, my original tweet was insulting to some even though that wasn’t my intention. I stick with original summary of the problem as I see it. >We need more experimentalists! Do more experiments!< And, as said before, the ideal scientist does both, in silico work & experimental work
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Wonder how the 2022
@ISMEJournal
cover image was made? Preprint: our new workflow combines SIP, FISH, SEM, Raman & NanoSIMS – all on the same cell! - is online ! Work by
@SchaibleGeorge
@Kohtzarchaeota
&
@EMSLscience
John Cliff. Funded
@NASA
@MooreFound
1/7
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Many of you will have to teach this fall while taking care of children at home or worrying about the well-being of friends and family. If you need someone to cover a class for you remotely, feel free to contact me!
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3 months later, turns out I am hyperresponder. I didn’t have such low blood Phe since I was 10 years old (44 uM, 0.7 mg/dL) and under strict diet control. For 1st time since I am an adult I was instructed to eat more protein! Thank you
#biomarinpharmaceutical
😢😊
@pkuawareness
I have a personal story to share. This week was BIG for me. I received my first shot of an enzyme treatment of an inborn metabolic disease, phenylketonuria (PKU, ). If untreated, PKU eventually leads to
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When I see people post “hiring SEVERAL postdocs” I don’t understand. Like, I am very well funded and I could never do this. Neither do I have the money to pay several PDs, nor the mental or mentoring capacity. Do they just pay shite and not mentor them or what am I doing wrong?
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@DanielJGiguere
I think nanodrop might be the most overvalued instrument in our science and I don’t understand why soooo many people get one with their startup
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On Monday, Dr. Heidi Smith,
@BiofilmFirst
microscopy manager, & I placed an order for a
@LeicaMicro
SP8 with Stimulated Raman option. The 2 of us raised $1.15M for this. Once installed, this instrument will only be the 3rd commercial Stimulated Raman scope at a US university
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@RBReich
Yes, they are called hypocrites. It's sad and funny at the same time, when people refuse to wear masks because "the government shouldn't interfere with my body" fail to see the irony and conflict in their argument.
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Still accepting applications for a 3-yr postdoc position on Asgard archaea (eco)physiology in my lab! Covid has delayed the start date. If interested and you can start by Apr 2021, make sure to apply. Please share! Ad: . Learn more:
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I am looking forward to visiting Boston and give the
@HarvardMSI
lecture on Thursday! Email me if you wanna meet and chat!
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If any postdoc candidate wants to work in my lab via a Simons Postdoc Fellowship, please reach out! Our lab is going on a cruise to Guaymas and Pescadero basin in April and there will be a lot of fresh samples to work with to pursue your ideas.
The Simons Postdoctoral Fellowships in Marine Microbial Ecology are now open for applications! Read more at the link below. Deadline to apply: 5/7.
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Looks great in print😍! (Must the first time in 5 years or so that I hold a print issue in my hand)
@SchaibleGeorge
@ISMEJournal
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Looking for a postdoctoral researcher to work on Asgard archaea ecophysiology via a 3-year collaborative project funded by the
@SimonsFdn
! Please share widely.
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@HLockeSTEM
Leave a perfect lab notebook with cross references and back up all data in meaningful way another person can understand.
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@melonievaughn_
AfterI had a puerto rican 1st gen female undergrad who had 3 first/coauthored papers published/in prep, who had been affected by hurrican, proposed to work w Native American college for outreach NOT get it because of her GPA, I see the GRFP as a way to funnel money to ivy league
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Congratulations to a fantastic achievement
@yuhang_hank
for cultivating one of the missing links in Earth's biogeochemical cycles
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@Siolix1
@icy464
@RadLegalTakes
@HeatherWeaverDC
Those two statements are not mutually exclusive. That guy is clearly an irresponsible gun owner.
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Extracting DNA and sequencing 16S rRNA gene amplicons in field. Cool (hot) concept!
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Why do people study E. coli when they could be doing THIS!?
Just coming back from a sampling cruise in the West Antarctic Peninsula. Sampled surface seawater (10 m depth), soil, snow, ice and air with
@DrBradBrad
. Fantastic expedition and high temperatures the entire time... 😥
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It’s typically (probably not always) explained by high blood Phe levels. Anyways, thanks to
@biomarin
, this week I received the first shot of an enzyme (phenylalanine amino-lyase) that will start degrading Phe in my blood.
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My grad student
@SchaibleGeorge
’s SEM-FISH correlative microscopy image of multicellular magnetotactic bacteria will be the cover image of
@ISMEJournal
for 2022! 🎉
First issue of the
@ISMEJournal
showing the image I took of the multicellular magnetotactic bacteria I study. The image will grace the cover of the journal for the entire year of 2022! What an honor! Huge thanks to
@environmicrobio
for the opportunity!
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I am looking for a postdoctoral researcher to work on Asgard archaea ecophysiology via a 3-year project funded by
@SimonsFdn
@MooreFound
! If interested, please apply, even if you would only be able to join 2021. Finding right person for project > timing!
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For those interested, I updated my lab’s info slides on BONCAT, which included a list of all microbial ecology papers using BONCAT, with Luke and Heidi’s great new study! Download slides for teaching, talks, or just reviewing the literature here
Happy to share a new pub () out in
@ISMEJournal
using "Activity-based metagenomics" to decipher coalbed methane production, with Heidi Smith
@ElliottBarnhart
@HannahDoris12
Rex Malmstrom, Danielle Goudeau
@FieldsOMicrobes
(
@montanastate
@USGS
@jgi
)
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Thank you reviewer
#4
☺️ you made my day. "Of all proposals that I have read and evaluated this year (and the previous year, and so on), this is the one that falls most likely into the category “breakthrough” and belongs into the highest priority class."
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New REU program “Microbiology of Low Oxygen Ecosystems”
@montanastate
. Send us your students!
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THE go-to review for Raman applications in microbiology. Must read if you are interested in cutting-edge Next-Gen Physiology techniques that covers the foundation and highlights of Raman for microbiology research. Great read
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Are u an undergrad – or know an undergrad - interested in (paid) summer research on extremophiles in beautiful Bozeman MT? Apply for 1 of 3 REU program
@msuchembiochem
@MSU_MicroImmuno
@MSUExtremeBio
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@RC_Epi
What i hate even more is when a journal asks me to submit the figures separately and then creates a file of all submitted fils to send to reviewers. Had to upload 20 separate files just for review. Whyyyyyyyyyyyyyyyyyy?
@ISMEJournal
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Very proud of my grad student
@SchaibleGeorge
who just received a NASA FINESST (Future Investigators in NASA Earth & Space Science & Technology) award! Here, George is seen sampling for multicellular bacteria in Little Sippewissett salt marsh. Congrats to this great achievement!
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Two covers in one year!
@SchaibleGeorge
and
@Kohtzarchaeota
made a textbook cover based on our lab’s SIP-SEM-FISH workflow published in
@ISMEComms
. Funded by
@MooreFound
and
@NASA
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