Iban Ubarretxena
@Ubarretxena
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Joined September 2010
Saw the mcmjr1 trial. Staining is suboptimal. Controls were good. Perhaps some reconstitution in dmpc. Need to discuss what to try next.
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Fingers crossed. Don't expect crystals but just aggregates and vesicles will be great
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We will need about twenty 100 mL beakers (250 mL ones will do). I'll bring some from my lab.
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it should be 200 microM sodium azide, not mM as in the first tweet
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Make one liter of it at 10X , that is: 200 mM sodium phosphate pH 7.0 500 mM NaCl 20 mM MgCl2 100 mM EDTA 200 microM sodium azide
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Ralph please make dialysis buffer: 20 mM sodium phosphate pH 7.0 50 mM NaCl 2 mM MgCl2 1 mM EDTA 20 mM sodium azide
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We will have MCMJR1 for tomorrow's experiment, will pick it up in the morning and arrive at NYSBC around 10 am
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using dialysis cassettes and buttons in the traditional way. We will make the lipids together.
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Ralph and I will set up 2D crystallization together sometime next week. Will take MCMJR1 and will set up a few conditions
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Jose and Nathan need some experience with each target before they can guarantee delivery
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We have to start all over again. I want to do a 2 liter purification trial with all our targets before we commit to an schedule
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Jose and Nathan have worked very hard, including Saturday and the Monday holiday but we will not be able to deliver protein today
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The purification of MHJF from 48 liters was a disaster. We believe we have severe aggregation problems.
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Nicolas, your nysbc email account seems to over its quota
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