🌈 Leveraging FAST of The Twinkle Factory with click chemistry for à-la-carte fluorescent reporting in microbiome! Let them introduce Click-FAST, tfPoppy-alkyne, plBlaze-alkyne 🍭
A set of spectrally orthogonal fluorogen-activating fluorescent tags, greenFAST and redFAST, are compatible with two-color, live-cell imaging are now available from Arnaud Gautier's lab!
Finally published in Nat. Commun. Two new products launched: tfDart, a dark (!) chromophore, & tfFox-NP, an ultra-bright non-membrane permeant fluorogen
"An engineered multifunctional protein tag for advanced fluorescence imaging
Hela Benaissa et al.
bioRxiv. posted 31 January 2021, 10.1101/2021.01.29.428635
There are a lot of options for split fluorescent proteins - finding the right one can be challenging in a sea of choices! Check out our recent blog post to see some of the newest and most popular split fluorescent proteins in our repository.
Very happy to share a new approach we developed at
@institut_curie
/
@CNRS
with my colleague and friend Arnaud Gautier from
@Sorbonne_Univ_
/
@ENS_ULM
CATCHFIRE allows to induce and sense protein interaction using a reversible fluorogenic system. More here :
Introducing HapAmp, an in vivo gene amplification system that exploits haploinsufficiency to amplify copy number and deliver high level expression. 20-fold increase in monoterpene titre in a single engineering step!
Connaissez-vous Twinkle factory? Cette jeune entreprise est née
@ENS_ULM
et
@institut_curie
, au sein de
@psl_univ
, et propose des outils uniques pour l’imagerie de fluorescence. Suivez cette aventure initiée, dans notre département, par Arnaud Gautier et Ludovic Jullien.
Comprehensive wrap-up of protocols for
#FAST
and
#splitFAST
engineering in Yeast Surface Display of the Molecular Biology Protocols series by Lina El Hajji, Hela Benaissa, and Arnaud Gautier
@Sorbonne_Univ_
Find out how
@EuroBioImaging
Nodes and imaging facilities bring collaborations with industry to life, how they work on a daily basis and how to build lasting partnerships!
Register for our webinar on October 28th at 10am CEST