Olivier Borkowski
@O_Borkowski
Followers
6,349
Following
1,787
Media
1,162
Statuses
6,170
Tenured Scientist in Synthetic Biology (Paris Area) Cell-Free ⦁ SynBio ⦁ Bioengineering ⦁ Microbiology @INRAE_Micalis & @UnivParisSaclay
France
Joined October 2011
Don't wanna be here?
Send us removal request.
Explore trending content on Musk Viewer
Jets
• 103521 Tweets
Patriots
• 103473 Tweets
Wanni
• 97729 Tweets
Bills
• 95099 Tweets
Onyeka
• 91588 Tweets
Ravens
• 68849 Tweets
Semih
• 68612 Tweets
#خادم_الحرمين_الشريفين
• 64355 Tweets
#الملك_سلمان_بن_عبدالعزيز
• 63497 Tweets
Lamar
• 60089 Tweets
Bengals
• 58452 Tweets
Browns
• 51950 Tweets
Penaltı
• 48664 Tweets
Spurs
• 45468 Tweets
Watson
• 42359 Tweets
Hoca
• 39698 Tweets
Texans
• 32170 Tweets
Panthers
• 32103 Tweets
Caleb
• 28777 Tweets
Colts
• 27179 Tweets
Burrow
• 25396 Tweets
Buffalo
• 20274 Tweets
Jaguars
• 19691 Tweets
#BBUK
• 18704 Tweets
Jayden Daniels
• 17660 Tweets
Congratulations Kellyrae
• 15480 Tweets
Derrick Henry
• 14762 Tweets
Josh Allen
• 13548 Tweets
Pats
• 11726 Tweets
#سلامتك_يابوفهد
• 11479 Tweets
Pinned Tweet
I am thrilled to announce the release of our review:
"What remains from living cells in bacterial lysate-based cell-free systems"
co-authored by Léa Wagner,
@Matt_Jules
, and myself, now available online at
@CSB_Journal
.
To read the review:
5
49
178
The end of PCR machines?
Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics
19
570
2K
A biological camera that captures and stores images directly into DNA
3
113
481
Peptide sequencing via reverse translation of peptides into DNA
9
57
276
Cell-free expression of RuBisCO for ATP production in the synthetic cells
3
49
221
Automated Platform for the Plasmid Construction Process
0
50
194
A universal molecular control for DNA, mRNA and protein expression
0
45
194
Now in
@NatureComms
:
Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology
0
53
194
Decoupling Growth and Production by Removing the Origin of Replication from a Bacterial Chromosome
3
32
186
Bioengineering lipid-based synthetic cells for therapeutic protein delivery
2
28
188
Systematic discovery of protein interaction interfaces using AlphaFold and experimental validation
2
38
186
Review on:
Mathematical Models of Plasmid Population Dynamics
1
41
184
Engineering Bacteria for Environmental Release: Regulatory Challenges and Design Strategies
0
50
176
Genetically Encoded Fluorescent Biosensor for Rapid Detection of Protein Expression
2
39
173
The expanded CRISPR toolbox for constructing microbial cell factories
2
49
171
A three-node Turing gene circuit forms periodic spatial patterns in bacteria
0
38
166
A new era of Synthetic Biology - microbial community design
0
40
164
Using machine learning to enhance and accelerate synthetic biology
1
32
147
Our paper coupling active learning and cell-free is published in
@NatureComms
!!! Great work done with
@MathildeK25
, Agnès zettor,
@pandi_amir
,
@PaulSoudier
, Angelo Batista,
@jeanloupfaulon
in collaboration with Fabrice Agou team at
@institutpasteur
7
20
137
Microbial interactions in theory and practice: when are measurements compatible with models?
0
27
134
Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology
5
30
134
Milligrams to kilograms: making microbes work at scale
0
24
137
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design
1
37
133
PlasmidMaker: a Versatile, Automated, and High Throughput End-to-End Platform for
Plasmid Construction
0
32
132
Synthetic biology: Learning the way toward high-precision biological design
0
34
132
Efficient Natural Plasmid Transformation of Vibrio natriegens Enables Zero-capital Molecular Biology
0
31
125
Robust and tunable performance of a cell-free biosensor encapsulated in lipid vesicles
3
24
119
Advancing high-throughput screening systems for synthetic biology and biofoundry
0
34
118
Evidence of a possible multicellular life cycle in Escherichia coli
2
25
109
Legendary bacterial evolution experiment enters new era
2
21
114
Directed evolution of material-producing microorganisms
0
14
110
Cell-Free Gene Expression edited by
@smashty
@jewettlab_NU
full of interesting methods and protocols
0
30
111
“Metabolic burden” explained: stress symptoms and its related responses induced by (over)expression of (heterologous) proteins in Escherichia coli
0
32
110
Production of Rainbow Colorants by Metabolically Engineered Escherichia coli
0
24
110
Does ribosome composition maximize growth rate?
The role of RNA instability
0
22
109
Laboratory evolution reveals transcriptional mechanisms underlying thermal adaptation of Escherichia coli
1
21
109
A synthetic cell-free pathway for biocatalytic upgrading of one-carbon substrates
2
25
107
Synthetic co-cultures: novel avenues for bio-based processes
!
0
32
106
Cell-Free Translation Quantification via a Fluorescent Minihelix
0
18
106
The story behind the paper "Rapid development of AI-designed antimicrobial peptides via cell-free biosynthesis"
by
@pandi_amir
1
22
106
Bacteria can maintain rRNA operons solely on plasmids for hundreds of millions of years
0
21
104
Snowprint: a predictive tool for genetic biosensor discovery
0
23
104
Reduction-to-synthesis: the dominant approach to genome-scale synthetic biology
0
20
102
Very nice work from El karoui lab
@SynthSysEd
Growth-dependent heterogeneity in the DNA damage response in Escherichia coli
2
38
103
Control engineering and synthetic biology: working in synergy for the analysis and control of microbial systems
0
28
101
Minimization of the E. coli ribosome, aided and optimized by community science
0
16
99
Modular Golden Gate Assembly of Linear DNA Templates for Cell-free Prototyping
1
17
99
Evolutionary explanations for heterogeneous behavior in clonal bacterial populations
1
21
98
A library of reporters of the global regulators of gene expression of Escherichia coli
0
30
96
Emerging Designs and Applications for Biomembrane Biosensors
0
16
97
Engineering activatable promoters for scalable and multi-input CRISPRa/i circuits
1
19
95
ATP biosensor reveals microbial energetic dynamics and facilitates bioproduction
0
19
94
Cell-free systems and genetic biosensors for accelerating enzyme and pathway prototyping
1
27
92
Modularized synthetic biology enabled intelligent biosensors
3
16
90
High-throughput single-cell transcriptomics of bacteria using combinatorial barcoding
0
21
90
Cell-free protein synthesis systems for vaccine design and production
0
27
90
Ribosome Pool Engineering Increases Protein Biosynthesis Yields
0
29
86
Autotrophic growth of E. coli is achieved by a small number of genetic changes
1
22
89
Have a look at our last bioarxiv paper coupling machine learning and cell-free:
5
26
89
Discovery of antibiotics that selectively kill metabolically dormant bacteria
0
21
89
Ligify: Automated genome mining for ligand-inducible transcription factors
1
19
87
SynBiopython: an open-source software library for Synthetic Biology
3
24
87
Optimizing protein expression in the One-Pot PURE system: insights into reaction composition and translation efficiency
0
17
85
Life by design: Crafting the future through synthetic biology | Science | AAAS
0
22
84
Colony pattern development of a synthetic bistable switch
0
16
84
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology
0
16
83
Advancing in vivo reprogramming with synthetic biology
0
9
82
Rapid, multiplexed, and enzyme-free nucleic acid detection using programmable aptamer-based RNA switches
0
16
81
Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time
0
33
80
Feedback between stochastic gene networks and population dynamics enables cellular decision making
1
14
82
Strategies in engineering sustainable biochemical synthesis through microbial systems
2
12
81
Circular Single-Stranded DNA: Discovery, Biological Effects, and Applications
0
25
81
An operator-based expression toolkit for Bacillus subtilis enables fine-tuning of gene expression and biosynthetic pathway regulation
0
14
81
Synthetic biology advances towards a bio-based society in the era of artificial intelligence
0
23
78
ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets
0
11
79
Trade-off Between Resistance and Persistence in High Cell Density Escherichia Coli Cultures
0
10
80
Machine learning identifies key metabolic reactions in bacterial growth on different carbon sources
0
21
80
Autotrophic growth of E. coli is achieved by a small number of genetic changes
0
24
79
A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
0
28
77
Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield
0
20
79
From T. Hwa lab:
Cellular perception of growth rate and the mechanistic originof bacterial growth law
0
17
78
Dynamics of bacterial operons during genome-wide stresses
0
16
79