Heterogeneity of late endosome/lysosomes shown by multiplexed DNA-PAINT imaging | Journal of Cell Biology | Rockefeller University Press

RT @cohenlaboratory: Preprint alert! Led by Shannon Rhoads, we used 🌈🔬 to elucidate the #organelle signatures of #neurons and #astrocytes a…

RT @aliceyting: APEX peroxidase is a versatile tool for proximity labeling and electron microscopy (EM) but its requirement for toxic H2O2…

RT @EricTopol: A substantial reduction of #Alzheimers disease associated with semaglutide (Ozempic) in > 1 million people with Type 2 diab…

Arc mediates intercellular tau transmission via extracellular vesicles | bioRxiv

Machine-guided design of cell-type-targeting cis-regulatory elements | Nature

@AbellJonny With Ophir’s help + a CZI team of PIs we have now fixed the silencing and localization issue of dCas9 fusions. Paper is under review now but can share the plasmid with you.

@AbellJonny One thing to keep in mind for safe harbors is that integration of concatemers of the donor plasmid are not uncommon, and one needs to screen for these with long range PCR. Could account for differences in expression across clones. Jax is working on a solution to this for iNDI.

@christlet @AbellJonny @NathanShaner @joachimgoedhart I can vouch for the miRFO670nano3 version. Impressive performance in the context of the split nbAlfa system that they describe (including low signal absent alfa-tagged target). Though not nearly as bright/photostable as halo/JFX dyes.

PLD3 and PLD4 synthesize S,S-BMP, a key phospholipid enabling lipid degradation in lysosomes - ScienceDirect

RT @ShorterLab: An ANXA11 P93S variant dysregulates TDP‐43 and causes corticobasal syndrome - Snyder - 2024 - Alzheimer's & Dementia - Wile…

Cell-Type Resolved Protein Atlas of Brain Lysosomes Identifies SLC45A1-Associated Disease as a Lysosomal Disorder | bioRxiv

@lindsey__young What a clever way to tackle a vexing problem - congrats to you and the team!

RT @OscarWilkins16: Our paper on cryptic splicing-controlled protein expression, which we hope will inspire safer and more adventurous gene…

Our take on striking new findings from the Ryskeldi-Falcon lab on hybrid protein filaments in disease. I suspect this will be the tip of the iceberg, and there are additional implications for normal cell biology from their study.

Pervasive mislocalization of pathogenic coding variants underlying human disorders: Cell

Grateful to the Kissick Family Foundation and Milken Institute for this joint award with Len Petrucelli to study the molecular origins of FTD