RT @PabloINik: CIFR (Clone–Integrate–Flip-out–Repeat): A toolset for iterative #genome and pathway #engineering of Gram-negative #bacteria—…

RT @MichelePartipi3: Amazing tool for multiple cycles of genetic engineering of Gram negative bacteria developed by @FilFederici and France…

RT @PabloINik: Leveraging engineered #Pseudomonas putida minicells for bioconversion of organic acids into short-chain methyl ketones by @K…

We hope this thread is helpful to everyone interested in synthetic biology and metabolic engineering, or simply curious about what our group has been up to in 2024. Have some relax in this break to charge your energies back! 🎉

And also Meliawati led this work on Paenibacillus polymyxafor isobutanol production by genes KO and KI, optimizing NADPH regeneration, and identifying key metabolic bottlenecks, offering insights for further biotechnological applications of this bacterium

Touching on new bugs, here @enricoorsi and @dellavallesimo led the work for creating a new CRISPR/Cas toolkit for the emerging C1 platform C. necator, showing unprecedented efficiencies and turnover times for gene deletions

And in this chapter, w/ @DeMaria_SynBio they outline use of L-threonine aldolases (LTAs) from E.coli and P.putida to efficiently synthesize 4-F-L-threonine in vitro + protocols for adapting these activities to produce other fluorometabolites in vivo 10.1016/bs.mie.2024.02.016

Going back to P. putida, this minireview w/ @vdlorenzo_CNB and @PabloINik traces the evolution of KT2440 from a model organism for biodegradation studies to a SynBio #chassis, highlighting its adaptability and importance in various applications

When talking #biosensors, it is important to understand that their construction and validation is not easy. Here, @enricoorsi & @mirbachh demonstrated an improved end-to-end workflow for building a suite of sensors to measure glyoxylate and glycolate

Whereas here @Javi_HernanSan developed a whole-cell #biosensor for co-culture setups that integrates #fluorescent reporters and synthetic auxotrophies, enabling detection of multiple molecules, including plastic degradation and environmental analysis.

Talking about #biosensors, here @FiorellaMasotti developed one for #glyphosate detection by engineering A. tumefaciens with an optimized phnG promoter, enabling detection in the 0.25–50 μM range in soil and water samples

@FavoinoGiusi demonstrates here how to increase malonyl-CoA availability in P. putida using biosensor-informed engineering, and enhancing polymer production through a non-canonical pathway to boost the output of malonyl-CoA-dependent biosynthetic processes

Here are the fluoro masters @DeMaria_SynBio & @ManuelNietoDom1 presenting a protocol for synthesizing 4-F-L-threonine using L-threonine aldolases from E. coli or P. putida, highlighting the potential of F-amino acids for new chemistries and applications

Then @enricoorsi & @Javi_HernanSan reason how by using noncanonical redox cofactors in C1-bioprocesses can enhance product yields and enable challenging redox reactions, overcoming limitations of natural cofactors, contributing to a circular carbon economy

We start with a review led by @erayubozkurt describing strategies for isolating, creating, & characterizing enzyme variants optimized for bioproduction, including high-throughput screening and automated systems for developing novel biocatalysts

New paper from the group developing a cutting edge genome editing tool for Cupriavidus necator! With @enricoorsi, @copa_bio, @dellavallesimo leading the work and including @NicoC_MicSynBio @PabloINik Chek below form more info!